Urease and urea amidolyase: Determination of activity in liverworts
نویسندگان
چکیده
منابع مشابه
Structure and function of urea amidolyase
Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) a...
متن کاملThe Determination of Urea in Urine by the Urease Method
Marshall’s1 urease method, in the modification proposed by Van Slyke and Cullen, is apparently the most accurate method available for the determination of urea. The method has, however, not yet attained the maximal degree of accuracy to be expected from volumetric processes in general. From the published figures of Van Slyke and Cullen, their modification appears to yield duplicates agreeing wi...
متن کاملSelective determination of urea using urease immobilized on ZnO nanowires
Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50-130 nm diameters and ~1 μm in lengths were used in an enzymebased urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two line...
متن کاملPurification and properties of the urea amidolyase from Candida utilis.
Urea amidolyase was purified to homogeneity from extracts of Candida utilis. The purification involves protamine sulfate precipitation, ammonium sulfate precipitation, polyethylene glycol precipitation, Sepharose 6B gel filtration, DEAE-cellulose column chromatography, and hydroxylapatite column chromatography. The final preparation is pure as judged by disc-gel electrophoresis. The molecular w...
متن کاملLeaf urea metabolism in potato. Urease activity profile and patterns of recovery and distribution of (15)N after foliar urea application in wild-type and urease-antisense transgenics.
The influence of urease activity on N distribution and losses after foliar urea application was investigated using wild-type and transgenic potato (Solanum tuberosum cv Désirée) plants in which urease activity was down-regulated. A good correlation between urease activity and (15)N urea metabolism (NH(3) accumulation) was found. The general accumulation of ammonium in leaves treated with urea i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Phytochemistry
سال: 1973
ISSN: 0031-9422
DOI: 10.1016/0031-9422(73)80386-3